- Protein A/G
Protein A/G is a recombinant fusion protein that combines IgG binding domains of both
Protein Aand Protein G. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50,460 daltons. The binding of Protein A/G is less dependent upon pH than Protein A, but otherwise has the additive properties of Protein A and G.
Protein A/G binds to all subclasses of human IgG, making it useful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G also binds to all subclasses of mouse IgG but does not bind mouse IgA, IgM or serum albumin. [Sikkema, J.W.D. (1989) Amer. Biotech. Lab, 7, 42.] This allows Protein A/G to be used for purification and detection of mouse monoclonal IgG antibodies, without interference from IgA, IgM and
serum albumin. Mouse monoclonal antibodies commonly have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G. [Eliasson, M., et al. (1988) J. Biol. Chem. 263, 4323-4327.] Protein A/G also has been used for purification of macaque IgG [Chackerian, B., et al. (2004) J. Virol. 78, 4037-4047.]
Other antibody binding proteins
In addition to Protein A/G, other immunoglobulin-binding bacterial proteins such as
Protein A, Protein Gand Protein Lare all commonly used to purify, immobilize or detect immunoglobulins. Each of these immunoglobulin-binding proteins has a different antibody binding profile in terms of the portion of the antibody that is recognized and the species and type of antibodies it will bind.
Wikimedia Foundation. 2010.
Look at other dictionaries:
Protein S — is a vitamin K dependent plasma glycoprotein synthesized in the liver. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b. FunctionThe best characterized function of Protein S is its… … Wikipedia
Protein A — is a 40 60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus . It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two component system… … Wikipedia
Protein L — is a 36,000 dalton immunoglobulin binding protein isolated from the bacteria Peptostreptococcus magnus . Unlike Protein A and Protein G, which bind to the Fc region of immunoglobilins (antibodies), Protein L binds antibodies through light chain… … Wikipedia
Protein C — Vorhandene Strukturdaten … Deutsch Wikipedia
Protein-C — Vorhandene Strukturdaten: 1aut … Deutsch Wikipedia
Protein Z — protein Name = protein Z caption = width = HGNCid = 9460 Symbol = PROZ AltSymbols = EntrezGene = 8858 OMIM = 176895 RefSeq = NM 003891 UniProt = P22891 PDB = ECnumber = Chromosome = 13 Arm = q Band = 34 LocusSupplementaryData = Protein Z is a… … Wikipedia
Protein G — is an immunoglobulin binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65 kDa (G148 protein G) and a 58 kDa (C40 protein G)  cell surface protein that has found… … Wikipedia
Protein — Pro te*in, n. [Gr. prw^tos first: cf. prwtei^on the first place.] (Physiol. Chem.) any polymer of an amino acid joined by peptide (amide) bonds. Most natural proteins have alpha amino acids as the monomeric constituents. All classical enzymes are … The Collaborative International Dictionary of English
Protein-S — Vorhandene Strukturdaten: 1z6c Gr … Deutsch Wikipedia
Protein S — Vorhandene Strukturdaten … Deutsch Wikipedia