Hybridoma


Hybridoma

Hybridoma are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, B-cells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell membranes more permeable. The fused hybrid cells (called hybridomas), being cancer cells, will multiply rapidly and indefinitely and will produce large amounts of the desired antibodies. They have to be selected and subsequently cloned by limiting dilution. Supplemental media containing Interleukin-6 (such as briclone) are essential for this step.

Selection occurs via culturing the newly fused primary hybridoma cells in selective-media, specifically media containing 1x concentration HAT for roughly 10-14 days. After using HAT it is often desirable to use HT containing media. Cloning occurs after identification of positive primary hybridoma cells. Clone by limited dilution. While some may believe that IL-6 is essential for this step, it is not necessary to add that expensive supplement, rather use 50% heat-inactivated FBS for the first week. Add 10% FBS DMEM to the clone culture plate after screening for single colony wells.

External links

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* http://www.cancer.gov/cancertopics/understandingcancer/immunesystem/Slide36


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Look at other dictionaries:

  • hybridoma — hybridoma. См. гибридома. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • hybridoma — [hī΄bri dō′mə] n. [ HYBRID + (MYEL)OMA] a tissue culture consisting of cancer cells fused to lymphocytes to mass produce a specific antibody …   English World dictionary

  • Hybridoma — A cell hybrid resulting from the fusion of a cancer cell and a normal lymphocyte (a type of white blood cell). The hybridoma is immortal in the laboratory and makes the same products as its parent cells forever. * * * A tumor of hybrid cells used …   Medical dictionary

  • Hybridoma — Abb.1: (1) Immunisation einer Maus; (2) Isolation von B Zellen aus der Milz; (3) Kultivierung von Myelomazellen; (4) Fusionierung von B und Myelomzelle; (5) Selektion und Screening geeigneter Zelllinien; (6) Weiterverarbeitung bzw. Aufbewahrung… …   Deutsch Wikipedia

  • hybridoma — noun Date: 1978 a hybrid cell produced by the fusion of an antibody producing lymphocyte with a tumor cell and used to culture continuously a specific monoclonal antibody …   New Collegiate Dictionary

  • hybridoma — (hi brī do mah) A fast growing cell line produced by fusing a cancer cell (myeloma) to another cell, such as an antibody producing cell …   Dictionary of microbiology

  • hybridoma — A cell hybrid in which a tumour cell forms one of the original source cells. In practice, confined to hybrids between T or B lymphocytes and appropriate myeloma cell lines …   Dictionary of molecular biology

  • hybridoma — /huy bri doh meuh/, n., pl. hybridomas. Biotech. a hybrid cell made in the laboratory by fusing a normal cell with a cancer cell, usu. a myeloma or lymphoma, in order to combine desired features of each, as the ability of the cancer cell to… …   Universalium

  • hybridoma — noun A hybrid cell engineered from a lymphocyte and a tumour cell; used to generate monoclonal antibodies …   Wiktionary

  • hybridoma — hy·bri·do·ma …   English syllables


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