Protein G


Protein G

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) [1] cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G.

Other antibody binding proteins

In addition to Protein G, other immunoglobulin-binding bacterial proteins such as Protein A, Protein A/G and Protein L are all commonly used to purify, immobilize or detect immunoglobulins. Each of these immunoglobulin-binding proteins has a different antibody binding profile in terms of the portion of the antibody that is recognized and the species and type of antibodies it will bind.

References

Reflist http://www.jbc.org/cgi/reprint/266/1/399 Sjobring U, Bjorck L, Kastern W, Streptococcal protein G. Gene structure and protein binding properties, J Biol Chem. 1991 Jan 5;266(1):399-405


Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Protein S — is a vitamin K dependent plasma glycoprotein synthesized in the liver. In the circulation, Protein S exists in two forms: a free form and a complex form bound to complement protein C4b. FunctionThe best characterized function of Protein S is its… …   Wikipedia

  • Protein A — is a 40 60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus . It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two component system… …   Wikipedia

  • Protein A/G — is a recombinant fusion protein that combines IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50,460 daltons. The binding of Protein …   Wikipedia

  • Protein L — is a 36,000 dalton immunoglobulin binding protein isolated from the bacteria Peptostreptococcus magnus . Unlike Protein A and Protein G, which bind to the Fc region of immunoglobilins (antibodies), Protein L binds antibodies through light chain… …   Wikipedia

  • Protein C — Vorhandene Strukturdaten …   Deutsch Wikipedia

  • Protein-C — Vorhandene Strukturdaten: 1aut …   Deutsch Wikipedia

  • Protein Z — protein Name = protein Z caption = width = HGNCid = 9460 Symbol = PROZ AltSymbols = EntrezGene = 8858 OMIM = 176895 RefSeq = NM 003891 UniProt = P22891 PDB = ECnumber = Chromosome = 13 Arm = q Band = 34 LocusSupplementaryData = Protein Z is a… …   Wikipedia

  • Protein — Pro te*in, n. [Gr. prw^tos first: cf. prwtei^on the first place.] (Physiol. Chem.) any polymer of an amino acid joined by peptide (amide) bonds. Most natural proteins have alpha amino acids as the monomeric constituents. All classical enzymes are …   The Collaborative International Dictionary of English

  • Protein-S — Vorhandene Strukturdaten: 1z6c Gr …   Deutsch Wikipedia

  • Protein S — Vorhandene Strukturdaten …   Deutsch Wikipedia


Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.