Protein electrophoresis
In
chemistry andmedicine , protein electrophoresis (a.k.a.Immunoelectrophoresis ) is a method of analysing a mixture ofprotein s by means ofgel electrophoresis , mainly inblood serum (blood plasma is not suitable). Before the widespread use of electrophoresis gels, protein electrophoresis was performed on paper (sometimes in combination withpaper chromotography ).Interpretation
There are two classes of
blood protein s:serum albumin andglobulin . They are generally equal in proportion, butalbumin is much smaller and lightly negatively charged, leading to an accumulation of albumin on the electrophoretic gel. A small band before albumin representstransthyretin (also named prealbumin). Some forms of medication or body chemicals can cause their own band, usually small. Abnormal bands (spikes) are seen inmonoclonal gammopathy of undetermined significance andmultiple myeloma , and are useful in the diagnosis of these conditions.The
globulin s are classified by their banding pattern (with their main representatives):* The "alpha" (α) band consists of two parts, 1 and 2:
** α1 - α1-antitrypsin, α1-acid glycoprotein.
** α2 -haptoglobin , α2-macroglobulin, α2-antiplasmin,ceruloplasmin .
* The "beta" (β) band -transferrin ,LDL , complement
* The "gamma" (γ) band -immunoglobulin (IgA, IgD, IgE, IgG and IgM).Paraprotein s (inmultiple myeloma ) appear in this band.See also
*
Gel electrophoresis
*Paraprotein
*Electrofocusing
*QPNC-PAGE
*Immunofixation External links
* [http://www3.interscience.wiley.com/cgi-bin/abstract/113343976/ABSTRACT Discontinuous native protein gel electrophoresis]
* [http://wolfson.huji.ac.il/purification/ Protein purification facility]
Wikimedia Foundation. 2010.
Look at other dictionaries:
protein electrophoresis — see serum protein e. and urine protein e … Medical dictionary
Serum protein electrophoresis — (SPEP) is a laboratory test that examines specific proteins in the blood called globulins. Blood must first be collected, usually into an airtight vial or syringe. Electrophoresis is a laboratory technique where the blood serum (the fluid portion … Wikipedia
serum protein electrophoresis — an assay for presence of M component in the serum, indicative of plasma cell dyscrasias … Medical dictionary
urine protein electrophoresis — an assay for presence of M component in the urine, indicative of plasma cell dyscrasias … Medical dictionary
Protein (disambiguation) — Protein is a class of biomolecules composed of amino acid chains.Protein may also refer to:Biochemistry* Antifreeze protein, class of polypeptides produced by certain fish, vertebrates, plants, fungi and bacteria * Conjugated protein, protein… … Wikipedia
Electrophoresis (disambiguation) — Electrophoresis is both a phenomenon and a technique used in many scientific disciplines. Interface and Colloid ScienceElectrophoresis is the most known effect from electrokinetic phenomena. It is a motion of dispersed particles relative to a… … Wikipedia
Electrophoresis — is the most well known electrokinetic phenomenon. It was discovered by Reuss in 1809. [Reuss, F.F. Mem.Soc.Imperiale Naturalistes de Moscow , 2, 327 1809] He observed that clay particles dispersed in water migrate under influence of an applied… … Wikipedia
Protein fingerprinting — can refer to any of the several methods for identifying or differentiating proteins: *Peptide mass fingerprinting, a method developed in 1993 that uses protein mass spectrometry following SDS PAGE *Older techniques using two dimensional… … Wikipedia
protein — proteinaceous /proh tee nay sheuhs, tee i nay /, proteinic, proteinous, adj. /proh teen, tee in/, n. 1. Biochem. any of numerous, highly varied organic molecules constituting a large portion of the mass of every life form and necessary in the… … Universalium
Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… … Wikipedia
