CHAPS is a
zwitterionic detergentused in the laboratory to solubilize biological macromolecules such as proteins. It is used as a non-denaturing solvent in the process of protein purificationand is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueoussolution due to their natively hydrophobiccellular environment. CHAPS can also be used in conjunction with nonionicdetergents such as Triton X-100.
CHAPS is an abbreviation for the chemical formula 3- [(3-Cholamidopropyl)dimethylammonio] -1-propanesulfonate (CAS No. 75621-03-3 ). A related detergent, called CHAPSO, has the same basic chemical structure with an additional
hydroxyl functional group; its full chemical name is 3- [(3-Cholamidopropyl)dimethylammonio] -2-hydroxy-1-propanesulfonate. Both detergents have low light absorbance in the ultravioletregion of the electromagnetic spectrum, which is useful for laboratory workers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.
* [http://www.merckbiosciences.co.uk/html/CBC/ASB_detergents.htm Merck Biosciences: CHAPS]
* [http://www.freepatentsonline.com/4372888.html Free Patents Online: Nondenaturing zwitterionic detergents: United States Patent 4372888]
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