A gene library is a
populationof organisms, each of which carries a DNAmolecule that was inserted into a cloning vector. Ideally, all of the cloned DNA moleculesrepresent the entire genomeof the organism. A genelibrary is also called " gene bank". This term also represents the collection of all of the vectormolecules, each carrying piece of the chromosomalDNA of the organism, prior to the insertion of these molecules into the population of the host cell.
Types of Gene Libraries
# Plasmid libraries: Have a capacity of 10 kb.
# Phage libraries: Have a capacity of 23 kb.
# Cosmid libraries: Have a capacity of 45 kb.
# Yeast artificial chromosome (YAC) libraries: Have a capacity of 1000 kb.
Creating a library
DNA moleculesof an organismin interest are isolated. The DNA moleculesare then partially digested by an endonucleaserestriction enzyme. Sometimes, the DNA moleculesare digested to different lengths of time in order to ensure that all the geneshave been digested to manageable sizes. The digested DNA molecules are run on agarose electrophoresisfor which a suitable range of lengths of DNApieces are isolated and ligated to vector plasmids. Statistically, 99% of the geneswill be incoporated into the plasmids. The plasmidsthen can be taken up by suitable hosts.The hosts are kept in liquid media and can be frozen at -80°C for a long period of time. Usually the hosts are bacteriathat do not contain any plasmidsso as to be sensitive to antibiotics.
The process of subdividing
genomic DNAinto clonable element and inserting them into host is called creating a library, a clone bank or a gene bank. A complete library of host cell will contain all of the genomic DNA of the source organism.
* [http://www.biotecnika.googlepages.com/gene_library.html Gene library]
Wikimedia Foundation. 2010.
Look at other dictionaries:
gene library — See: library … Glossary of Biotechnology
gene library — A haphazard assembly of cloned DNA fragments inside of a vector which may contain genetic information about a species … Medical dictionary
Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… … Wikipedia
Gene Okerlund — Ring name(s) Mean Gene Okerlund Billed height 5 ft 10 in (1.78 m) … Wikipedia
Gene trapping — is a high throughput approach that is used to introduce insertional mutations across the mammalian genome. It is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene… … Wikipedia
Gene Arnold — (b. July 11, 194X), nicknamed Giant Gene by his fans at record hops and dances, is a radio and television personality from Philadelphia. Early career Arnold began his musical career in the 1950s, appearing on American Bandstand as a teenager in… … Wikipedia
Gene Gauntier — Nombre real Genevieve Liggett Nacimiento 17 de mayo de 1885 Kansas City (Misuri), Estados Unidos Fall … Wikipedia Español
Gene Yang — Gene Luen Yang (born in Alameda, California) Contra Costa Times staff (January 29 2006). Sunday A E big calendar . Contra Costa Times , Pg. F4 ] is an American comics artist whose graphic novel American Born Chinese was named a 2006 finalist for… … Wikipedia
Gene Amdahl — Gene Myron Amdahl (born November 16, 1922) is a Norwegian American computer architect and hi tech entrepreneur, chiefly known for his work on mainframe computers at International Business Machines (IBM) and later his own companies, especially… … Wikipedia
Gene Dub — Born September 20, 1943 Edmonton, Alberta, Canada Nationality Canadian Work Practice Dub Architects Gene Dub (born September 20, 1943) is a Canadian architect and for … Wikipedia