Phenylalanine

Phenylalanine
Identifiers
CAS number 150-30-1 (DL) YesY, 63-91-2 (L)
PubChem 994
ChemSpider 5910 YesY
UNII 8P946UF12S YesY
DrugBank DB00120
KEGG D00021 YesY
ChEBI CHEBI:58095 N
ChEMBL CHEMBL301523 YesY
Jmol-3D images Image 1
Properties
Molecular formula C9H11NO2
Molar mass 165.19 g mol−1
Acidity (pKa) 1.83 (carboxyl), 9.13 (amino)[1]
Supplementary data page
Structure and
properties
n, εr, etc.
Thermodynamic
data
Phase behaviour
Solid, liquid, gas
Spectral data UV, IR, NMR, MS
 N (verify) (what is: YesY/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Phenylalanine (abbreviated as Phe or F)[2] is an α-amino acid with the formula C6H5CH2CH(NH2)COOH. This essential amino acid is classified as nonpolar because of the hydrophobic nature of the benzyl side chain. L-Phenylalanine (LPA) is an electrically neutral amino acid, one of the twenty common amino acids used to biochemically form proteins, coded for by DNA. The codons for L-phenylalanine are UUU and UUC. Phenylalanine is a precursor for tyrosine, the monoamine signaling molecules dopamine, norepinephrine (noradrenaline), and epinephrine (adrenaline), and the skin pigment melanin.

Phenylalanine is found naturally in the breast milk of mammals. It is used in the manufacture of food and drink products and sold as a nutritional supplement for its reputed analgesic and antidepressant effects. It is a direct precursor to the neuromodulator phenylethylamine, a commonly used dietary supplement.

Contents

Other biological roles

L-Phenylalanine is biologically converted into L-tyrosine, another one of the DNA-encoded amino acids. L-tyrosine in turn is converted into L-DOPA, which is further converted into dopamine, norepinephrine (noradrenaline), and epinephrine (adrenaline). The latter three are known as the catecholamines.

Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.

DLPA RXN Phenylalanine.GIF

In plants

Phenylalanine is the starting compound used in the flavonoid biosynthesis. Lignan is derived from phenylalanine and from tyrosine. Phenylalanine is converted to cinnamic acid by the enzyme phenylalanine ammonia-lyase.[3]

Phenylketonuria

The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine. Individuals with this disorder are known as "phenylketonurics" and must regulate their intake of phenylalanine. A (rare) "variant form" of phenylketonuria called hyperphenylalaninemia is caused by the inability to synthesize a coenzyme called biopterin, which can be supplemented. Pregnant women with hyperphenylalaninemia may show similar symptoms of the disorder (high levels of phenylalanine in blood) but these indicators will usually disappear at the end of gestation. Individuals who cannot metabolize phenylalanine must monitor their intake of protein to control the buildup of phenylalanine as their bodies convert protein into its component amino acids.

Phenylketonurics often use blood tests to monitor the amount of phenylalanine in their blood. Lab results may report phenylalanine levels in different units, including mg/dL and umol/L. One mg/dL of phenylalanine is approximately equivalent to 60 umol/L.

A non-food source of phenylalanine is the artificial sweetener aspartame. This compound, sold under the trade names "Equal" and "NutraSweet", is metabolized by the body into several chemical byproducts including phenylalanine. The breakdown problems phenylketonurics have with protein and the attendant build up of phenylalanine in the body also occurs with the ingestion of aspartame, although to a lesser degree. Accordingly, all products in Australia, the U.S. and Canada that contain aspartame must be labeled: "Phenylketonurics: Contains phenylalanine." In the UK, foods containing aspartame must carry ingredient panels that refer to the presence of "aspartame or E951" [4] and they must be labeled with a warning "Contains a source of phenylalanine." These warnings are specifically placed to aid individuals who suffer from PKU so that they can avoid such foods.

Geneticists have recently sequenced the genome of macaques. Their investigations have found "some instances where the normal form of the macaque protein looks like the diseased human protein" including markers for PKU.[5]

D- and DL-phenylalanine

The stereoisomer D-phenylalanine (DPA) can be produced by conventional organic synthesis, either as a single enantiomer or as a component of the racemic mixture. It does not participate in protein biosynthesis although it is found in proteins in small amounts - particularly aged proteins and food proteins that have been processed. The biological functions of D-amino acids remain unclear although some, such as D-phenylalanine, may have pharmacological activity.[citation needed]

DL-Phenylalanine (DLPA) is marketed as a nutritional supplement for its supposed analgesic and antidepressant activities. DL-Phenylalanine is a mixture of D-Phenylalanine and L-Phenylalanine. The reputed analgesic activity of DL-phenylalanine may be explained by the possible blockage by D-phenylalanine of enkephalin (endorphin) degradation by the enzyme carboxypeptidase A.[6] The mechanism of DL-phenylalanine's supposed antidepressant activity may be accounted for by the precursor role of L-phenylalanine in the synthesis of the neurotransmitters norepinephrine and dopamine. Elevated brain levels of norepinephrine and dopamine are thought to have an antidepressant effect. Also, as DL-phenylalanine inhibits endorphin degradation, this leads to an inhibition of GABA release in the ventral tegmental neurons (in the midbrain), which results in greater dopamine release. This can explain the analgesic effects following ingestion, D-Phenylalanine is absorbed from the small intestine and transported to the liver via the portal circulation. A small amount of D-phenylalanine appears to be converted to L-phenylalanine. D-Phenylalanine is distributed to the various tissues of the body via the systemic circulation. It appears to cross the blood-brain barrier less efficiently than L-phenylalanine, and so a small amount of an ingested dose of D-phenylalanine is excreted in the urine without penetrating the central nervous system.

Commercial synthesis

L-Phenylalanine is produced for medical, feed, and nutritional applications, such as Aspartame, in large quantities by utilizing the bacterium Escherichia coli, which naturally produces aromatic amino acids like phenylalanine. The quantity of L-phenylalanine produced commercially has been increased by genetically engineering E. coli, such as by altering the regulatory promoters or amplifying the number of genes controlling enzymes responsible for the synthesis of the amino acid.[7]

History

The genetic codon for phenylalanine was first discovered by J. Heinrich Matthaei and Marshall W. Nirenberg in 1961. They showed that by using m-RNA to insert multiple uracil repeats into the genome of the bacterium E. coli, they could cause the bacterium to produce a polypeptide consisting solely of repeated phenylalanine amino acids. This discovery helped to establish the nature of the coding relationship that links information stored in genomic nucleic acid with protein expression in the living cell.

See also

  • Hyperphenylalanemia

References

  1. ^ Dawson, R.M.C., et al., Data for Biochemical Research, Oxford, Clarendon Press, 1959.
  2. ^ IUPAC-IUBMB Joint Commission on Biochemical Nomenclature. "Nomenclature and Symbolism for Amino Acids and Peptides". Recommendations on Organic & Biochemical Nomenclature, Symbols & Terminology etc. http://www.chem.qmul.ac.uk/iupac/AminoAcid/. Retrieved 2007-05-17. 
  3. ^ Nelson, D. L.; Cox, M. M. "Lehninger, Principles of Biochemistry" 3rd Ed. Worth Publishing: New York, 2000. ISBN 1-57259-153-6.
  4. ^ Aspartame, Food Standards Agency.
  5. ^ Scientists decode macaque genome, BBC News, 13 April 2007.
  6. ^ Christianson DW, Mangani S, Shoham G, Lipscomb WN. "Binding of D-phenylalanine and D-tyrosine to carboxypeptidase A." Journal of Biological Chemistry 1989 Aug 5;264(22):12849-53. PMID 2568989.
  7. ^ Sprenger, George A. (May 2007). "Aromatic amino acids". Amino acid biosynthesis: pathways, regulation and metabolic engineering (1st ed.). Springer. pp. 106–113. ISBN 978-3540485957 

External links



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Look at other dictionaries:

  • Phénylalanine — L ou S( ) phénylalanine D ou R …   Wikipédia en Français

  • Phenylalanine — Phénylalanine Phénylalanine Formule topologique …   Wikipédia en Français

  • phénylalanine — [ fenilalanin ] n. f. • 1897; de phényl et alanine ♦ Biochim. Composé aromatique, l un des vingt acides aminés constituants des protéines. ● phénylalanine nom féminin Acide aminé essentiel, cyclique, très répandu, obtenu par hydrolyse de… …   Encyclopédie Universelle

  • phenylalanine — phenylalanine. См. фенилаланин [Фен]. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • phenylalanine — [fen΄əl al′ə nēn΄, fē΄nəlal′ə nēn΄] n. an essential amino acid, C6H5CH2CH(NH2)COOH, occurring in proteins: see AMINO ACID …   English World dictionary

  • phenylalanine — /fen l al euh neen , nin, feen /, n. Biochem. a crystalline, water soluble, essential amino acid, C6H5CH2CH(NH2)COOH, necessary to the nutrition of humans and most animals, obtained chiefly from egg white or skim milk. Abbr.: Phe; Symbol: F Also… …   Universalium

  • phenylalanine — 2 Amino 3 phenylpropionic acid; the l isomer is one of the common amino acid s in proteins; a nutritionally essential amino acid. p. ammonia lyase a nonmammalian enzyme that catalyzes the conversion of l p. to trans cinnamate and ammonia; it has… …   Medical dictionary

  • phenylalanine — n. an essential amino acid that is readily converted to tyrosine. Blockade of this metabolic pathway gives rise to phenylketonuria, which is associated with abnormally large amounts of phenylalanine and phenylpyruvic acid in the blood and… …   The new mediacal dictionary

  • phenylalanine — fenilalaninas statusas T sritis chemija formulė C₆H₅CH₂CH(NH₂)COOH santrumpa( os) Phe, F atitikmenys: angl. phenylalanine rus. фенилаланин ryšiai: sinonimas – 2 amino 3 fenilpropano rūgštis …   Chemijos terminų aiškinamasis žodynas

  • phenylalanine — noun Etymology: International Scientific Vocabulary Date: circa 1883 an essential amino acid C9H11NO2 that is converted in the normal body to tyrosine …   New Collegiate Dictionary


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