Protein methods are the techniques used to study
List of Protein Methods
There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified.
*conceptual translation- many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA sequence. See
*site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
**insertion of protein tags such as the
His-tag. See also: Green fluorescent protein.
*evolutionary; analysis of sequence changes in different species using software such as
*Proteins that are involved in human diseases can be identified by matching
alleles to disease and other phenotypes using methods such as calculation of LOD scores.
* Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination Methods,
Bradford protein assay
* Concentrating Protein Solutions
** Gel Electrophoresis Under denaturing conditions
** Gel Electrophoresis Under non-denaturing conditions
** 2D Gel Electrophoresis
Protein structure prediction
Protein structural alignment
Peptide mass fingerprinting
Ligand binding assay
**heavy isotope labeling
**radioactive isotope labeling
*"Protein Methods", 2nd Edition by Daniel M. Bollag, Michael D. Rozycki and Stuart J. Edelstein (1996) Published by Wiley Pblishers ISBN 0-471-11837-0.
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