Force spectroscopy is a dynamic analytical technique that allows the study of the mechanical properties of single
polymer molecules or proteins, or individual chemical bonds. It is performed by pulling on the system under scrutiny with controlled forces. As a single-moleculetechnique, as opposed to typical ensemble spectroscopies, it allows a researcher to determine properties of the particular molecule under study. In particular, rare events such as conformational change, which are masked in an ensemble, may be observed.
The name "force
spectroscopy", although widely used in the scientific community, is somewhat misleading, because there is no true matter-radiation interaction. Force spectroscopy measures the behaviour of a molecule under stretching or torsional mechanical force. In this way a great deal has been learned in recent years about the mechanochemical coupling in the enzymes responsible for musclecontraction, transport in the cell, energy generation (F1-ATPase), DNAreplication and transcription (polymerases), DNA unknotting and unwinding (topoisomerases and helicases), and so on.
There are many ways to accurately manipulate single molecules. Prominent among these are optical or magnetic tweezers and atomic-force-microscope (AFM) cantilevers. In all of these techniques, a biomolecule, such as protein or DNA, or some other biopolymer has one end bound to a surface and the other to a force sensor. The force sensor is usually a micrometre-sized bead or a cantilever, whose displacement can be measured to determine the force.
Atomic force microscope cantilevers
Molecules adsorbed on a
surfaceare picked up by a microscopic tip (nanometres wide) that is located on the end of an elastic cantilever. In a more sophisticated version of this experiment (Chemical Force Microscopy) the tips are covalently functionalized with the molecules of interest. A piezoelectriccontroller then pulls up the cantilever. If some force is acting on the elastic cantilever (for example because some molecule is being stretched between the surface and the tip), this will deflect upward (repulsive force) or downward (attractive force). According to Hooke's law, this deflection will be proportional to the force acting on the cantilever. Deflection is measured by the position of a laserbeam reflected by the cantilever. This kind of set-up can measure forces as low as 10 pN (10-11 N), and cannot achieve much better resolution only because of thermal noise. The so-called force curve is the graph of force (or more precisely, of cantilever deflection) versus the piezoelectric position on the Z axis. An ideal Hookean spring, for example, would display a straight diagonal force curve.Typically, the force curves observed in the force spectroscopy experiments consist of a contact (diagonal) region where the probe contacts the sample surface, and a non-contact region where the probe is off the sample surface. When the restoring force of the cantilever exceeds tip-sample adhesion force the probe jumps out of contact, and the magnitude of this jump is often used as a measure of adhesion force or rupture force. In general the rupture of a tip-surface bond is a stochastic process; therefore reliable quantification of the adhesion force requires taking multiple individual force curves. The histogram of the adhesion forces obtained in these multiple measurements provides the main data output for force spectroscopy measurement.
Quite often researchers repeat the measurements as a function of the bond loading rate. The resulting graph of the average rupture force as a function of the loading rate is called the "force spectrum" and forms the basic dataset for the "dynamic force spectroscopy". In the ideal case of a single sharp energy barrier for the tip-sample interactions the dynamic force spectrum will show a linear increase of the rupture force as function of a logarithm of the loading rate. The slope of the line is equal to the , where is the distance from the energy minimum to the transition state.
Another technique that has been gaining ground for single molecule experiments is the use of
optical tweezersfor applying mechanical forces on molecules. A strongly focused laserbeam has the ability to catch and hold particles (of dielectric material) in a size range from nanometers to micrometers. The trapping action of optical tweezers results from the dipole or optical gradient force on the dielectric sphere. The technique of using a focused laser beam as an atom trap was first applied in 1984 at Bell laboratories. Until then experiments had been carried out using oppositely directed lasers as a means totrap particles. Later experiments, at the same project at Belllaboratories and others since, showed damage-free manipulation on cells using an infrared laser. Thus, the ground was made for biological experiments with optical trapping.
Each technique has its own advantages and disadvantages. For example, AFM cantilevers, can measure angstrom-scale, millisecond events and forces larger than 10 pN. While glass microfibers cannot achieve such fine spatial and temporal resolution, they can measure piconewton forces. Optical tweezers allow the measurement of piconewton forces and nanometer displacements which is an ideal range for many biological experiments. Magnetic tweezers can measure femtonewton forces, and additionally they can also be used to apply torsion.
Common applications of force spectroscopy are measurements of
polymerelasticity, especially biopolymers such as RNAand DNA. Another exciting biophysical application of polymer force spectroscopy is on proteinunfolding. Modular proteins can be adsorbed to a goldor (more rarely) mica surfaceand then stretched. The sequential unfoldingof modules is observed as a very characteristic sawtooth pattern of the force vs elongation graph; every tooth corresponds to the unfolding of a single protein module (apart from the last that is generally the detachment of the protein molecule from the tip) A lot of information about protein elasticity and protein unfolding can be obtained by this technique. This is even more interesting if we consider the fact that many proteins in the living cell must face mechanical stress.
The other main application of force spectroscopy is the study of
mechanical resistanceof chemical bonds. In this case generally the tip is functionalized with a ligand that binds to another molecule bound to the surface. The tip is pushed on the surface, allowing for contact between the two molecules, and then retracted until the newly formed bond breaks up. The force at which the bond breaks up is measured. Since mechanical breaking is a kinetic, stochastic process, the breaking force is not an absolute parameter, but it is a function of both temperature and pulling speed. Low temperatures and high pulling speeds correspond to higher breaking forces. By careful analysis of the breaking force at various pulling speeds, it is possible to map the energylandscape of the chemical bond under mechanical force. This is leading to interesting results in the study of antibody- antigen, protein-protein and even protein-living cell interaction.
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