Cell-free protein synthesis
Cell-free protein synthesis (also called
in-vitro protein synthesis), is the production of protein without the use of living cells.
The first elucidation of a
codonwas done by Marshall Nirenbergand Heinrich J. Matthaeiin 1961 at the National Institutes of Health. They used a cell-free system to translate a poly-uracil RNA sequence (or UUUUU... in biochemical terms) and discovered that the polypeptide they had synthesized consisted of only the amino acid phenylalanine. They thereby deduced from this poly-phenylalanine that the codon UUU specified the amino-acid phenylalanine [neur] . Extending this work, Nirenberg and his coworkers were able to determine the nucleotide makeup of each codon
Cell-free systems in common use.
Wikimedia Foundation. 2010.
Look at other dictionaries:
cell-free protein synthesis — See: in vitro translation … Glossary of Biotechnology
Cell-free protein array — technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This method of synthesizing protein microarrays overcomes the many obstacles and challenges faced by traditional methods of… … Wikipedia
cell — cell1 cell like, adj. /sel/, n. 1. a small room, as in a convent or prison. 2. any of various small compartments or bounded areas forming part of a whole. 3. a small group acting as a unit within a larger organization: a local cell of the… … Universalium
protein — proteinaceous /proh tee nay sheuhs, tee i nay /, proteinic, proteinous, adj. /proh teen, tee in/, n. 1. Biochem. any of numerous, highly varied organic molecules constituting a large portion of the mass of every life form and necessary in the… … Universalium
Protein — Proteins are large organic compounds made of amino acids arranged in a linear chain and joined together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues. The sequence of amino acids in a protein is defined by … Wikipedia
Cell (biology) — Allium cells in different phases of the cell cycle … Wikipedia
Cell nucleus — HeLa cells stained for the cell nucleus DNA with the Blue Hoechst dye. The central and rightmost cell are in interphase, thus their entire nuclei are labeled. On the left, a cell is going through mitosis and its DNA has condensed ready for… … Wikipedia
Protein microarray — A protein microarray, sometimes referred to as a protein binding microarray, is a piece of glass on which different molecules of protein have been affixed at separate locations in an ordered manner thus forming a microscopic array. These are used … Wikipedia
Protein kinase A — In cell biology, Protein kinase A (PKA) refers to a family of enzymes whose activity is dependent on cellular levels of cyclic AMP (cAMP). PKA is also known as cAMP dependent protein kinase (EC 18.104.22.168). Protein kinase A has several functions… … Wikipedia
Cell membrane — Illustration of a Eukaryotic cell membrane The cell membrane or plasma membrane is a biological mem … Wikipedia