(= low stringency; high stringency; stringency wash)
In nucleic acid hybridization, the labelled probe is used to label matching sequences by base-pairing. Unbound probe is removed though a series of stringency washes. Low stringency washing (low temperature, high ionic strength), allows some mismatching of probe and target, and thus allows the detection of similar sequences at some cost in specificity. By contrast, high stringency conditions allow only closely matching sequence to remain base-paired.

Dictionary of molecular biology. 2004.

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  • stringency — UK US /ˈstrɪndʒənsi/ noun [U] ► a situation in which a law, test, etc. is extremely severe or limiting and must be obeyed: the stringency of sth »The statute prohibits the adoption of environmental protections laws that exceed the stringency of… …   Financial and business terms

  • Stringency — Strin gen*cy (str[i^]n jen*s[y^]), n. The quality or state of being stringent. [1913 Webster] …   The Collaborative International Dictionary of English

  • stringency — index austerity, rigor, severity Burton s Legal Thesaurus. William C. Burton. 2006 …   Law dictionary

  • stringency — (n.) 1844, from STRINGENT (Cf. stringent) + CY (Cf. cy) …   Etymology dictionary

  • stringency — [strin′jən sē] n. pl. stringencies the quality or state of being stringent; strictness; severity …   English World dictionary

  • stringency — /strin jeuhn see/, n., pl. stringencies. 1. stringent character or condition: the stringency of poverty. 2. strictness; closeness; rigor: the stringency of school discipline. 3. tightness; straitness: stringency in the money market. [1835 45;… …   Universalium

  • stringency — [[t]strɪ̱nʤ(ə)nsi[/t]] N UNCOUNT: supp N Financial stringency is a situation in which a government or person does not have much money or is trying not to spend much. [FORMAL] In times of financial stringency it is clear that public expenditure… …   English dictionary

  • stringency — Reaction conditions (notably temperature, salt concentration and pH) that affect the annealing process of single stranded DNA or RNA to make double stranded DNA or RNA, or DNA/RNA hybrids. At high stringency, duplexes form only between strands… …   Glossary of Biotechnology

  • stringency — /ˈstrɪndʒənsi/ (say strinjuhnsee) noun (plural stringencies) 1. stringent character or condition. 2. strictness; closeness; rigour. 3. tightness; straitness: stringency in the money market …   Australian English dictionary

  • stringency — stringent ► ADJECTIVE ▪ (of regulations or requirements) strict, precise, and exacting. DERIVATIVES stringency noun stringently adverb. ORIGIN from Latin stringere draw tight …   English terms dictionary

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