polyacrylamide gel electrophoresis
- (= PAGE)Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place, and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that non-covalently associating subunit polypeptides migrate independently, and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. See also SDS-PAGE.
Dictionary of molecular biology. 2004.
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polyacrylamide gel electrophoresis — polyacrylamide gel electrophoresis. См. электрофорез в полиакриламидном геле. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
polyacrylamide gel electrophoresis — (PAGE) gel electrophoresis using a polymerized polyacrylamide matrix to separate molecules on the basis of size, charge, or both; usually used to separate proteins or sequence nucleic acids. Gels are usually discontinuous (see disc e.) but may be … Medical dictionary
polyacrylamide gel electrophoresis — Abbreviation: PAGE Ubiquitous method for separating nucleic acids and proteins on the basis of their molecular size. The method relies on the migration through an inert matrix (polyacrylamide gel) of electrically charged molecules as a result of… … Glossary of Biotechnology
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SDSâ€“polyacrylamide gel electrophoresis — (SDS PAGE) a type of polyacrylamide gel electrophoresis in which the anionic detergent sodium dodecyl sulfate (SDS) is used to denature the sample proteins into linear monomers, rendering their charge proportional to their length so that… … Medical dictionary
sodium dodecyl sulphate polyacrylamide gel electrophoresis — Abbreviation: SDS PAGE A widely employed electrophoretic method for the separation of proteins from biological samples. The sodium dodecyl sulphate gives a uniform charge density to the surface of proteins or nucleic acids, so that their rate of… … Glossary of Biotechnology
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polyacrylamide gel — n hydrated polyacrylamide that is used esp. to provide a medium for the suspension of a substance to be subjected to gel electrophoresis … Medical dictionary