multiple cloning site


multiple cloning site

Dictionary of molecular biology. 2004.

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  • Multiple cloning site — A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to 20) restriction sites a standard feature of engineered plasmids.[1] Restriction sites within an MCS are typically unique, occurring only …   Wikipedia

  • Multiple Cloning Site — Ein Polylinker oder eine Multiple Cloning Site (MCS) ist ein DNA Abschnitt in einem Vektor, dessen Sequenz direkt hintereinander verschiedene Schnittstellen für Restriktionsendonukleasen enthält. Diese ermöglicht ein flexibles Klonieren, da aus… …   Deutsch Wikipedia

  • multiple cloning site — Abbreviation: MCS See: polylinker …   Glossary of Biotechnology

  • Cloning vector — The pGEX 3x plasmid is a popular cloning vector. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the… …   Wikipedia

  • Multiple displacement amplification — (MDA) is a non PCR based DNA amplification technique. This method can rapidly amplify minute amount of DNA samples to reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template and DNA… …   Wikipedia

  • Cloning — For the cloning of human beings, see Human cloning. For other uses, see Cloning (disambiguation). The sea anemone, Anthopleura elegantissima in process of cloning Cloning in biology is the process of producing similar populations of …   Wikipedia

  • polycloning site — (= multiple cloning site; MCS) Region of a phage or plasmid vector that has been engineered to contain a series of restriction sites that are usually unique within the entire vector. This makes it particularly easy to insert or excise (subclone)… …   Dictionary of molecular biology

  • Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

  • MCS — Multiple Chemical Sensitivity (Medical » Physiology) *** Multiple Cloning Site (Medical » Human Genome) ** Multiple Chemical Sensitivities (Medical » Human Genome) * Media Convergence Server (Computing » Networking) * Mesoscale Convective System… …   Abbreviations dictionary

  • Restriction enzyme — Glossary Restriction …   Wikipedia


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