Lowry assay

Lowry assay
One of the most commonly used assays for protein content - the paper describing it is said to be the most frequently cited in the biological literature. Depends upon the interaction of Folin-Ciocalteau reagent with tyrosine or phenylalanine. Proteins that are deficient in these amino acids (eg. collagen) will be underestimated and for this reason the Bradford assay is often preferred.

Dictionary of molecular biology. 2004.

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  • Lowry — may refer to:PeopleIrish families of the Lowry, Lowery, or Lavery name descend from the O Labhradha, a sept of the province of Ulster anciently. In the later days they are found in County Down near Moira.Three branches of the name are of record.… …   Wikipedia

  • Assay — An assay is an analysis done to determine: {{}}The presence of a substance and the amount of that substance. Thus, an assay may be done for example to determine the level of thyroid hormones in the blood of a person suspected of being hypothyroid …   Medical dictionary

  • Lowry protein assay — The Lowry protein assay, or Lowry Method, is a biochemical test to measure the amount of protein in a sample.Copper(II) ion in alkaline solution reacts with protein to form complexes, which react with the Folin phenol reagent, a mixture of… …   Wikipedia

  • Assay — An assay is a procedure where a property or concentration of an analyte is measured.There are numerous types of assays, such as an antigen capture assay, bioassay, competitive protein binding assay, crude oil assay, four point assay, immunoassay …   Wikipedia

  • Lowry-Test — Die Proteinbestimmung nach Lowry ist eine Methode, um Proteine quantitativ zu bestimmen.[1] Sie beruht auf zwei Reaktionen: Der erste Schritt beruht auf der Biuretreaktion, nämlich auf der Bildung eines blau violetten, quadratisch planaren… …   Deutsch Wikipedia

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  • Lowry — Oliver H., U.S. biochemist, *1910. See L. Folin assay, L. protein assay. R. Brian, 20th century Irish medical geneticist in Canada. See Coffin L. syndrome …   Medical dictionary

  • Oliver H. Lowry — Oliver Howe Lowry (July 18, 1910 – June 29, 1996) was an American biochemist. He is best remembered for devising the Lowry protein assay. Lowry was the youngest of a family of five children. His father was a teacher and later an administrator in… …   Wikipedia

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  • Enzyme assay — Beckman DU640 UV/Vis spectrophotometer. Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Contents …   Wikipedia

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