ligase amplification reaction

ligase amplification reaction
Method for detecting small quantities of a target DNA, with utility similar to PCR. It relies on DNA ligase to join adjacent synthetic oligonucleotides after they have bound the target DNA. Their small size means that they are destabilised by single base mismatches, and so form a sensitive test for the presence of mutations in the target sequence.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

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