- insertional mutagenesis
Generally, mutagenesis of DNA by the insertion of one or more bases. Specific examples: (1) oncogenesis by insertion of a retrovirus adjacent to a cellular proto-oncogene;(2) a strategy of mutagenesis with transposons. After a round of transposition, progeny are screened by PCR, with transposon- and gene-specific primers, for the proximity of the transposon sequence to the gene of interest. As PCR can only produce products up to 1-2 kb, a large fraction of progeny identified as positive by PCR will have a transposon close enough to the gene to inactivate or otherwise alter its pattern of expression.
Dictionary of molecular biology. 2004.
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Insertional mutagenesis — is mutagenesis of DNA by the insertion of one or more bases. Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab. Contents 1 Signature tagged mutagenesis 2 … Wikipedia
insertional mutagenesis — noun (genetics) a mutation caused by the insertion of exogenous DNA into a genome • Topics: ↑genetics, ↑genetic science • Hypernyms: ↑mutagenesis … Useful english dictionary
viral-induced insertional mutagenesis — viral induced insertional mutagenesis. См. вирусный инсерционный мутагенез. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
Mutagenesis — (pronounced /mjuːtəˈdʒɛnɪsɪs/) is a process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation. It may occur spontaneously in nature, or as a result of exposure to mutagens. It can also be… … Wikipedia
mutagenesis — noun an event capable of causing a mutation • Hypernyms: ↑cause • Hyponyms: ↑insertional mutagenesis • Part Holonyms: ↑mutation, ↑genetic mutation, ↑chromosomal mutation … Useful english dictionary
mutagenesis — 1. Production of a mutation. 2. Production of genetic alteration through use of chemicals or radiation. cassette m. the production of mutants within a region (often bounded by unique restriction sites) by the use of synthetic oligonucleotides… … Medical dictionary
Signature tagged mutagenesis — Often abbreviated to STM, Signature Tagged Mutagenesis is a genetic technique used to study gene function. Recent advances in genome sequencing have allowed us to catalogue a large variety of organisms genomes, but the function of the genes they… … Wikipedia
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Transposable element — A bacterial DNA transposon Transposable elements (TEs) are sequences of DNA that can move or transpose themselves to new positions within the genome of a single cell. The mechanism of transposition can be either copy and paste or cut and paste .… … Wikipedia
Gene therapy — using an Adenovirus vector. A new gene is inserted into an adenovirull. If the treatment is successful, the new gene will make a functional protein. Gene therapy is the insertion, alteration, or removal of genes within an individual s cells and… … Wikipedia