- glutathione S transferase
Enzyme that will couple glutathione to a xenobiotic as the first step in removal. Now very commonly used as a fusion with a gene of interest that is being expressed in a bacterial system. The fusion construct can be purified easily from lysate by passage down a glutathione affinity column, the purified construct then being eluted with glutathione. The GST can then be cleaved proteolytically from the protein of interest though often the complete fusion protein can be used.
Dictionary of molecular biology. 2004.
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