glutathione S transferase


glutathione S transferase
Enzyme that will couple glutathione to a xenobiotic as the first step in removal. Now very commonly used as a fusion with a gene of interest that is being expressed in a bacterial system. The fusion construct can be purified easily from lysate by passage down a glutathione affinity column, the purified construct then being eluted with glutathione. The GST can then be cleaved proteolytically from the protein of interest though often the complete fusion protein can be used.

Dictionary of molecular biology. 2004.

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  • Glutathione S-transferase Mu 1 — (gene name GSTM1) is a human glutathione S transferase. PBB Summary section title = summary text = Cytosolic and membrane bound forms of glutathione S transferase are encoded by two distinct supergene families. At present, eight distinct classes… …   Wikipedia

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  • glutathione S-transferase — A family of enzymes involved in metabolism and in making toxic compounds less harmful to the body …   English dictionary of cancer terms

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  • glutathione — A tripeptide of glycine, l cysteine, and l glutamate, with l glutamate having an isopeptide bond with the amino moiety of l cysteine. G. has a wide variety of roles in a cell; it is the most prevalent non protein thiol. G. disulfide (GSSG)… …   Medical dictionary

  • Glutathion-S-Transferase — Enzymklassifikation EC, Kategorie …   Deutsch Wikipedia


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