gel retardation assay
- (= mobility shift assay)Test for interaction between molecules by looking for a change in gel electrophoretic mobility. For example, to assay for levels of a transcription factor, cell extracts are incubated with a radiolabelled oligonucleotide corresponding to the recognition sequence of the transcription factor, and run on an agarose gel. Most of the radiolabel will run quickly through the gel, but any radioactivity that is retarded is presumably caused by DNA/transcription factor interaction.
Dictionary of molecular biology. 2004.
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