Antisense RNA


Antisense RNA

Antisense RNA (aRNA) is single-stranded RNA that is complementary to a messenger RNA (mRNA) strand transcribed within a cell. Antisense RNA may be introduced into a cell to inhibit translation of a complementary mRNA by base pairing to it and physically obstructing the translation machinery. This effect is therefore stoichiometric. An example of naturally occurring mRNA antisense mechanism is the hok/sok system of the E.coli R1 plasmid. Antisense RNA has long been thought of as a promising technique for disease therapy; the only such case to have reached the market is the drug fomivirsen. One commentator has characterized antisense RNA as one of "dozens of technologies that are gorgeous in concept, but exasperating in [commercialization] ".Citation
last=DePalma |first=Angelo |date=August 2005 |title=Twenty-Five Years of Biotech Trends
periodical=Genetic Engineering News |publisher=Mary Ann Liebert |volume=25 |issue=14 |pages=1, 14–23
url=http://www.genengnews.com/articles/chitem.aspx?aid=1005&chid=0 |accessdate=2008-08-17 |issn=1935-472X
] Generally, antisense RNA still lack effective design, biological activity, and efficient route of administration. [ [http://mct.aacrjournals.org/cgi/content/full/1/5/347 Antisense Oligonucleotides: Basic Concepts and Mechanisms] Nathalie Dias and C. A. Stein. Columbia University, New York, New York 10032 ]

Historically, the effects of antisense RNA have often been confused with the effects of RNA interference, a related process in which double-stranded RNA fragments called small interfering RNAs trigger catalytically mediated gene silencing, most typically by targeting the RNA-induced silencing complex (RISC) to bind to and degrade the mRNA. Attempts to genetically engineer transgenic plants to express antisense RNA instead activate the RNAi pathway, although the processes result in differing magnitudes of the same downstream effect, gene silencing. Well-known examples include the Flavr Savr tomato and two cultivars of ringspot-resistant papaya. Sanders RA, Hiatt W. (2005). Tomato transgene structure and silencing. "Nat Biotechnol" 23(3):287-9. PMID 15765076] Chiang C, Wang J, Jan F, Yeh S, Gonsalves D (2001). "Comparative reactions of recombinant papaya ringspot viruses with chimeric coat protein (CP) genes and wild-type viruses on CP-transgenic papaya". "J Gen Virol" Pt 11): 2827-36. PMID 11602796]

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Look at other dictionaries:

  • antisense RNA — antisense RNA. См. антисмысловая РНК. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Antisense-RNA — Translationshemmung durch Antisense RNA Die Antisense RNA (aRNA) ist eine einzelsträngige RNA, die komplementär zur Messenger RNA (mRNA) ist. Die mRNA wird vom nicht kodierenden Strang (Matrizenstrang) der DNA transkribiert. Wird auch der… …   Deutsch Wikipedia

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  • R1162-like plasmid antisense RNA — The copy number of the broad host range plasmid R1162 is controlled by the amounts of two proteins, encoded by cotranscribed genes comprising a region of the plasmid called RepI. RepI is negatively regulated by a 75 base RNA that is complementary …   Wikipedia

  • RNA interference — (RNAi) is a mechanism that inhibits gene expression at the stage of translation or by hindering the transcription of specific genes. RNAi targets include RNA from viruses and transposons (significant for some forms of innate immune response), and …   Wikipedia

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