- Extractable nuclear antigens
Extractable Nuclear Antigens are soluble cytoplasmic and nuclear components that are
antibodytargets with over 100 different antigens described. The main 6 used in immunological laboratories for detection are Ro, La, Sm, RNP, Scl-70and Jo1, which are screened for by Ouchterlony double immuno diffusiontechniques and confirmed by Immuno blotting. Antibodies to these antigens have particular associations with various connective tissue disorders.
anti-nuclear antibodytests, they have a speckled pattern.cite web |url=http://library.med.utah.edu/WebPath/IMMHTML/IMM005.html |title=Immunopathology |format= |work= |accessdate=]
ENAs originally referred to proteins found in a saline extract of cell nuclei. Its components have since been more clearly identified and in fact include many cytoplasmic molecules. The misnomer however has stuck. These proteins are intimately associated with various RNA molecules and are thus called ribonucleoproteins, but the nomenclature used for them is often a source of confusion, Sm, Ro and La were named after the first 2 letters of the surnames of the patients in whom they were first found. Two proteins associated with Sjogren's Syndrome were independently descrbied as antigens A and B, but are now known to be identical to Ro and La respectively. i.e. SS-A = Ro and SS-B = La.
ENA 4 is a grouping of antibodies often used to screen for
mixed connective tissue disease(MCTD), Sjögren's syndromeand systemic lupus erythematosusand commonly is composed of four tests: [ENA 4 test (QUANTA Lite) product sheet. inovadx.com. URL: [http://www.inovadx.com/Products/di_pdfs/708555/628555rEnglish.pdf http://www.inovadx.com/Products/di_pdfs/708555/628555rEnglish.pdf ENA] . Accessed on: November 5, 2007.]
*anti-Sm (for SLE)
*anti-RNP (for MCTD)
*anti-La (for Sjögren's)
*anti-Ro (for Sjögren's)
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