Ham's tissue culture medium

Ham's tissue culture medium

Ham's tissue culture medium is a growth medium for mammalian cells containing in amounts dissolved in 1 liter of triple distilled water:

L-Arginine 211 mg Biotin 0.024 mg L-Histidine 21 mg Calcium pantothenate 0.7 mg L-Lysine 29.3 mg Choline chloride 0.69 mg L-Methionine 4.48 mg i-inositol 0.54 mg L-Phenylalanine 4.96 mg Niacinamide 0.6 mg L-Tryptophan 0.6 mg Pyridoxine hydrochloride 0.2 mg L-Tyrosine 1.81 mg Riboflavin 0.37 mg L-Alanine 8.91 mg Thymidine 0.7 mg Glycine 7.51 mg Cyanocobalamin 1.3 mg L-Serine 10.5 mg Sodium pyruvate 110 mg L-Threonine 3.57 mg Lipoic acid 0.2 mg L-Aspartic acid 13.3 mg CaCl2 44 mg L-Glutamic acid 14.7 mg MgSO4.7H2O 153 mg L-Asparagine 15 mg Glucose 1.1 g L-Glutamine 146.2 mg NaCl 7.4 g L-Isoleucine 2.6 mg KCl 285 mg L-Leucine 13.1 mg Na2HPO4 290 mg L-Proline 11.5 mg KH2PO4 83 mg L-Valine 3.5 mg Phenol red 1.2 mg L-Cysteine 31.5 mg FeSO4 0.83 mg Thiamine hydrochloride 1 mg CuSO4.5H2O 0.0025 mg Hypoxanthine 4 mg ZnSO4.7H2O 0.028 mg Folic acid 1.3 mg NaHCO3 1.2 g

See also

*Cell biology
*DMEM Dulbecco's Modified Eagle's Medium
*Eagle's minimal essential medium (a minimum ingredient cell culture medium)
*Fermentation (food)
*GMEM (Glasgow's Minimal Essential Medium)
*Murashige and Skoog medium (Plant cell culture medium)
*RPMI (Roswell Park Memorial Institute medium), for lymph cells


* [http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/Media.html The Nutrient Requirements of Cells]

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